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. 2023 May 24;150(10):dev201522. doi: 10.1242/dev.201522

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© 2023. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Pseudotime analysis of published scRNA-seq data from human embryos to establish the order of appearance of hypoblast markers. (A) Human preimplantation embryo UMAP embedding (Radley et al., 2022) sub-setted down to samples involved during bifurcation to epiblast/hypoblast populations. Left panel: cell type labels defined by Stirparo et al. (2018). Right panel: pseudotime along the hypoblast branch. (B) NANOG and SOX2 distinguish hypoblast and epiblast populations. kNN, k-nearest neighbours. (C) Fitting a logistic curve to the smoothed gene expression against hypoblast pseudotime delineates hypoblast gene activation. (D) Overlays of smoothed gene expression onto UMAP confirms ordered upregulation specific to the hypoblast.