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Generation of CRISPR/Cas9 edited hESCs carrying the intron 5 mutation in CHMP2B. (A) Schematic representation of the CRISPR/Cas9 strategy used to target and modify the CHMP2B gene. (B) Summary of results from screening clonal lines upon genome editing. (C) Sanger sequencing chromatogram of clone B13 (left panel) and results of TOPO clones after analysis of 16 samples.
