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Validation of factors involved in NMD-coupled protein quality control. Factors of interest were individually depleted by sgRNA in K562 Zim3 CRISPRi cells expressing the indicated reporter. Displayed are the RFP:GFP ratios for the NMD2 (top) and control (bottom) reporters as determined by flow cytometry; see Fig. S5A,C,E for quantification. Similar results were obtained using reverse reporters as in Fig. S5B,D,F.
