Table 2.
Composition of stock solutions.
| Stock solution | Component | Quantity/L | Note |
|---|---|---|---|
| 10X MS Macrosalts | NH4NO3 | 16.5 g | First dissolve CaCl2·2H2O in distilled water at 70% of the volume to be prepared before adding the other ingredients and then add the remaining ingredients. Make up the vol and autoclave. Store at 4°C |
| KNO3 | 19 g | ||
| KH2PO4 | 1.7 g | ||
| MgSO4·7H2O | 3.7 g | ||
| CaCl2·2H2O | 4.4 g | ||
| 1000X MS Microsalts | MnSO4 * | 10 g | *If MnSO4 is not available use MnSO4·H2O (11.37 g), MnSO4·4H2O (15.48 g) or, MnSO4·7H2O (18.63 g). Sterilize by autoclaving and store at 4°C |
| H3Bo3 | 6.2 g | ||
| ZnSO4·2H2O | 5.8 g | ||
| KI | 0.8 g | ||
| Na2MoO4·2H2O | 0.25 g | ||
| CuSO4·5H2O | 0.025 g | ||
| CoCl·6H2O | 0.025 g | ||
| 10X MS | MgSO4 *7H2O | 3.70 g | Divide into 50 ml aliquots and store at -20°C #Store at 4°C ##Store at –20°C |
| MnSO4 *H2O | 169 mg | ||
| CaCl2 *2 H2O | 4.35 g | ||
| NH4NO3 | 16.5 g | ||
| KNO3 | 19.0 g | ||
| KH2PO4 | 1.7 g | ||
| H3BO3 | 62 mg | ||
| ZnSO4*7H2O | 86 mg | ||
| Fe (III) EDTA | 425 mg | ||
| Myo-Inositol | 1.0 g | ||
| CuSO4*5H2O [0.25 mg/ml]# | 1.0 ml | ||
| KI [7.5 mg/ml] # | 1.0 ml | ||
| CoCl2*6H2O [0.25 mg/ml)] # | 1.0 ml | ||
| Na2MoO4*2H2O [2.5 mg/ml] # | 1.0 ml | ||
| Nicotinic Acid [5.0 mg/ml]## | 1.0 ml | ||
| Pyridoxine (B6) [5.0 mg/ml] ## | 1.0 ml | ||
| Thiamine (B1) [1.0 mg/ml] ## | 1.0 ml | ||
| 1000X MS Vitamins/Inositol (-Glycine) | Thiamine.HCl | 100 mg | Sterilize by autoclaving and store at 4°C |
| Pyridoxine.HCI | 500 mg | ||
| Nicotinic acid | 500 mg | ||
| Inositol | 2.0 g | ||
| MS - FeNaEDTA | FeSO4.7H2O | 2.785g | Store at 4°C |
| Na2EDTA.2H2O | 3.725 g | ||
| 10X L7 Macrosalts | NH4NO3 | 2.5 g | Store at 4°C |
| KNO3 | 15.0 g | ||
| KH2PO4 | 2.0 g | ||
| MgSO4·7H2O | 3.5 g | ||
| CaCl2·2H2O | 4.5 g |