Figure 5.

Performance and simulation of metaRUpore in retrieving MAGs of the rare species within the TAD microbiome at increasing sequencing depths. (A) The number of DQ MAGs retrieved for rare species by metaRUpore and normal nanopore sequencing. (B) Significant promotion in the minimum relative abundance to get HQ MAGs by metaRUpore as compared to normal nanopore sequencing (P-value is shown). The theoretical minimum value at a given sequencing data size was calculated as the lowest relative abundance of a species with a genome size of 3 Mb to reach 30× genome coverage.