Table 3.

Iso-Seq data characterization and transcriptome completeness.

		Brain	Heart	Kidney	Liver	Lung	Muscle	Testis
Transcriptome	No. of CCS reads	847,617	253,468	723,158	1,087,892	1,061,936	125,633	527,678
	High-quality isoforms	73,422	80,600	45,097	47,491	28,508	16,078	44,605
	Low-quality isoforms	734	844	616	384	151	94	284
	No. of genes	10,449	10,448	9,063	10,924	6,564	4,772	9,613
	Mean gene length (bp)	24,193	20,119	16,350	15,125	18,528	17,397	17,415
	No. of CDS	27,449	28,747	25,790	27,202	13,551	8,447	23,009
	Mean CDS length (bp)	972	985	932	823	894	980	960
	No. of exons	231,169	222,791	235,989	194,325	108,084	69,859	184,794
	Mean exon length (bp)	246	248	223	225	221	224	209
	Mean no. of exons/mRNA	8.4	8.2	7.9	7.1	8.0	8.3	8.0
BUSCO	Complete (%)	56.80	57.50	48.30	49.40	38.30	31.20	49.3
	Single-copy (%)	40.30	39.50	33.60	34.70	31.1	27.00	34.6
	Duplicated (%)	16.50	18.00	14.70	14.70	7.20	4.20	14.70
	Fragmented (%)	2.90	2.10	2.60	3.20	2.00	1.10	2.30
	Missing (%)	40.30	40.60	49.10	47.40	59.70	67.70	48.40