Fig. 5.

Inhibition of MPXV replication by trifluridine and Adefovir dipivoxil (ADP). (A) HFFs were infected with MPXV-WA-2003-Fluc (luc + MPXV) under an early/late promoter (MOI = 0.01) and treated with indicated compounds at the indicated concentration for 24 h. Firefly luciferase activities were measured. The inhibition by AraC was normalized to 100. (B) MPXV-MA001 2022 isolate was added to cells at an MOI of 2 for 1 h. The virus was removed, cells were washed with PBS, and trifluridine or adefovir dipivoxil was added at 10 μM. Cells were harvested 24 h post-infection. AraC treatment was used as the positive control. Viral yields were titrated using a plaque assay on E6 cells. The plotted values represent the means of at least three repeats. Error bars represent standard deviation. *p ≤ 0.05; **0.001<p ≤ 0.01; ***0.0001<p ≤ 0.001; ****0.00001<p ≤ 0.0001; ns, not significant. DMSO was used as the vehicle for the compounds.