FIGURE 4.

Summaries of efficacies of species delimitation powers based on the p‐dist method across the 657 bp 3' mtCOI barcoding gene region in the cryptic B. tabaci and non‐tabaci species complex. The metabarcoding primer pair “wfly‐PCR‐F1/R1” (472 bp) was divided into four overlapping regions of 315 bp across a 50 bp sliding window size, and the 518 bp amplicon generated by the “wfly‐PCR‐F2/R2” primer pair was divided into five overlapping regions of 315 bp. We also assessed full amplicon lengths from both primer pairs (i.e., F1/R1: 472 bp; F2/R2: 518 bp, blue coloured cells). Cryptic species that could not be defined were represented by grey coloured cells, and sequences that were successfully defined into their respective species clades were shown by green cells. Dark grey cells indicated failed species delimitation by both primer pairs and full species delimitation will require the complete 657 bp barcoding gene. The number of clean mtCOI sequences in the database (db) of Kunz, Tay, Elfekih, et al. (2019) for each species analysed are indicated (total: 224 sequences)