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Live cell imaging of ATP evoked intracellular Ca2+ transients monitored by GCaMP variant sensors in HEK293T cells.
| jGCaMP8f | jGCaMP8f L27A |
jGCaMP8f F366H |
|
| ΔF/Fo (ATP) HEK293T | 2.4 ± 0.4 | 1.0 ± 0.1 | 9.0 ± 0.9 |
| ΔF/Fo ionomycin HEK293T | 3.2 ± 0.2 | 2.8 ± 0.2 | 11.8 ± 1.1 |
| Time to peak (s) (ATP) HEK293T |
1.4 ± 0.3 | 1.0 ± 0.1 | 1.8 ± 0.5 |
| t1/2 (decay) (s) (ATP) HEK293T | 11.5 ± 4.7 | 3.1 ± 1.1 | 7.3 ± 4.0 |
