FIGURE 3.

FcεRIγ has a dose‐dependent effect on receptor expression on the cell surface. Effect of varying amounts of FcεRIγ DNA on expression of MCL A, Mincle B, Dectin‐2 C, or DCIR D, following transfection of 293T cells. MFI–geometric mean fluorescence intensity. Significant differences from receptor alone are indicated. E, Effect of varying amounts of FcεRIγ DNA on expression of MCL transfected alone or together with Mincle or Dectin‐2. Significant increases over transfection with MCL together with an identical amount of FcεRIγ are indicated. F, Effect of varying amounts of FcεRIγ on expression of Mincle transfected alone or together with MCL. G, Effect of varying amounts of FcεRIγ on expression of Dectin‐2 transfected alone or together with MCL. Cells were transfected in triplicate for each experiment, and data shown are pooled from two independent experiments, normalized to MFI of the individual receptors transfected alone without FcεRIγ (arbitrarily set to 100). Statistical significance of varying FcεRIγ vector amount A‐D, was determined using one way ANOVA, with Dunnett correction for multiple comparisons. For comparison of receptor expression between groups E‐G, statistical significance was determined using multiple t‐tests with Holm–Sidak correction for multiple comparisons. *P < .05, **P < .01, ***P < .001