KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Goat anti-Scgb1a1 (clone T-18) | Santa Cruz Biotechnology Inc. | Cat# sc-9772; RRID: AB_2238819 |
| Rabbit anti-Scgb1a1 | Seven Hills Bioreagents | Cat# WRAB-CCSP; RRID: AB_451716 |
| Mouse anti-α-Actin (clone 1A4) | Santa Cruz Biotechnology Inc. | Cat# sc-3251; RRID: AB_262054 |
| Rabbit anti-FGF10 | Acris Antibodies | Cat# AP14882PU-N; RRID: AB_1752406 |
| Chicken anti-Green Fluorescent Protein | Aves Labs Inc. | Cat# GFP-1020; RRID: AB_10000240 |
| Rabbit anti-Cytokeratin 5 (clone EP1601Y) | Thermo Fisher Scientific | Cat# MA5-14473; RRID: AB_10979451 |
| Chicken anti-Keratin 5 | BioLegend | Cat# 905904; RRID: AB_2721743 |
| Rabbit anti-Mucin 5B (clone H-300) | Santa Cruz Biotechnology Inc. | Cat# sc-20119; RRID: AB_2282256 |
| Rabbit anti-BMP4 | Bioss Inc. | Cat# BS-1374R; RRID: AB_10857765 |
| Mouse anti-Shh (clone E–1) | Santa Cruz Biotechnology Inc. | Cat# sc-365112; RRID: AB_10709580 |
| Mouse anti-FoxJ1 | Thermo Fisher Scientific | Cat# 14-9965-82; RRID:AB_1548835 |
| Rabbit anti-red fluorescent protein | Rockland Immunochemicals | Cat# 600-401-379; RRID: AB_2209751 |
| Cy3 AffiniPure F(ab’)2 Fragment Donkey Anti-Goat IgG (H + L) | Jackson ImmunoResearch | Cat# 705-166-147; RRID: AB_2340413 |
| Cy3 AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG (H + L) | Jackson ImmunoResearch | Cat# 715-166-150; RRID: AB_2340816 |
| Cy3 AffiniPure F(ab’)2 Fragment Donkey Anti-Rabbit IgG (H + L) | Jackson ImmunoResearch | Cat# 711-166-152; RRID: AB_2313568 |
| Alexa Fluor 647 AffiniPure F(ab’)2 Fragment Donkey Anti-Goat IgG (H + L) | Jackson ImmunoResearch | Cat# 705-606-147; RRID: AB_2340438 |
| Alexa Fluor 647 AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG (H + L) | Jackson ImmunoResearch | Cat# 715-606-150; RRID: AB_2340865 |
| Alexa Fluor 647 AffiniPure F(ab’)2 Fragment Donkey Anti-Rabbit IgG (H + L) | Jackson ImmunoResearch | Cat# 711-606-152; RRID: AB_2340625 |
| Alexa Fluor 488 AffiniPure F(ab’)2 Fragment Donkey Anti-Chicken IgY (IgG) (H + L) | Jackson ImmunoResearch | Cat# 703-546-155; RRID: AB_2340376 |
| Alexa Fluor 488 AffiniPure F(ab’)2 Fragment Donkey Anti-Goat IgG (H + L) | Jackson ImmunoResearch | Cat# 705-546-147; RRID: AB_2340430 |
| Alexa Fluor 488 AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG (H + L) | Jackson ImmunoResearch | Cat# 715-546-150; RRID: AB_2340849 |
| Alexa Fluor 488 AffiniPure F(ab’)2 Fragment Donkey Anti-Rabbit IgG (H + L) | Jackson ImmunoResearch | Cat# 711-546-152; RRID: AB_2340619 |
| Chemicals, peptides, and recombinant proteins | ||
| Naphthalene | Sigma-Aldrich | Cat# 84679 |
| Antigen Unmasking Solution, Citrate-Based | Vector Labs | Cat# H-3000 |
| Tamoxifen | Millipore Sigma | Cat# T5648 |
| VECTASHIELD® Antifade Mounting Medium | Vector Labs | Cat# H-1000 |
| Bst LF polymerase | Biolabs Inc. | Cat# M0275L |
| Proteinase K | Thermo Fisher Scientific | Cat# 25530–049 |
| RNALater | Thermo Fisher Scientific | Cat# AM7021 |
| Critical commercial assays | ||
| Omega E.Z. RNA Total RNA Kit I | Omega Biotek | Cat #R6834-02 |
| Maxima First Strand cDNA Synthesis | Fisher Scientific | Cat# FERK1642 |
| Taqman Gene Expression Assays | Applied biosystems | Cat# 4369016 |
| Experimental models: Organisms/strains | ||
| Mouse: Fgfr2bf/f | De Moerlooze et al. | N/A |
| Mouse: B6;129S-Sox2tm1(cre/ERT2)Hoch/J (Sox2CreERT2) | The Jackson Laboratory | RRID:IMSR_JAX:017593 |
| Mouse: B6N.129S6(Cg)-Scgb1a1tm1(cre/ERT)Blh/J (Scgb1a1CreERT) | The Jackson Laboratory | RRID:IMSR_JAX:01625 |
| Mouse: Scgb1a1Cre | Ji et al., Simon and Mariani | N/A |
| Mouse: B6.129(Cg)-Gt(ROSA) 26Sortm4(ACTB-tdTomato,-EGFP)Luo/J (Rosa26-mTmG) | The Jackson Laboratory | RRID:IMSR_JAX:007676 |
| Mouse: B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J (Rosa26-tdTomato) | The Jackson Laboratory | RRID:IMSR_JAX:007909 |
| Mouse: Nkx2-1tm2.1(flpo)Zjh/J (Nkx2.1flpo) | The Jackson Laboratory | RRID:IMSR_JAX:028577 |
| Mouse: 129S4/SvJae-Fgfr1tm11.1Sor/J (Fgfr1Cerulean) | The Jackson Laboratory | RRID:IMSR_JAX:030708 |
| Mouse: 129S4/SvJae-Fgfr2tm2.1Sor/J (Fgfr2mCherry) | The Jackson Laboratory | RRID:IMSR_JAX:030710 |
| Mouse: Krt5CreERT2 | Van Keymeulen et al. | N/A |
| Mouse: Acta2CreERT2 | Wendling et al. | N/A |
| Mouse: Fgf10f/f | Urness et al. | N/A |
| Mouse: B6.129X1-Wnt7btm2Amc/J (Wnt7bf/f) | The Jackson Laboratory | RRID:IMSR_JAX:008467 |
| Mouse: B6.129P2-Lgr6tm2.1(cre/ERT)Cle/J (Lgr6CreERT2) | The Jackson Laboratory | RRID:IMSR_JAX:016934 |
| Mouse: B6;129-Shhtm2Amc/J (Shhf/f) | The Jackson Laboratory | RRID:IMSR_JAX:004293 |
| Mouse: Gli1tm3(cre/ERT2)Alj/J (Gli1CreERT2) | The Jackson Laboratory | RRID:IMSR_JAX:007913 |
| Mouse: Smotm2Amc/J (Smof/f) | The Jackson Laboratory | RRID:IMSR_JAX:004526 |
| Mouse: Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J (R26SmoM2) | The Jackson Laboratory | RRID:IMSR_JAX:005130 |
| Mouse: B6(SJL)-Piezo2tm1.1(cre)Apat/J (Piezo2Cre) | The Jackson Laboratory | RRID:IMSR_JAX:027719 |
| Oligonucleotides | ||
| Keratin 5 (K5) (qPCR primer) | Thermo Fisher Scientific | Mm01305291_g1 |
| p63 (Trp63) (qPCR primer) | Thermo Fisher Scientific | Mm00495788_m1 |
| Scgb1a1 (qPCR primer) | Thermo Fisher Scientific | Mm00442046_m1 |
| Muc5b (qPCR primer) | Thermo Fisher Scientific | Mm00466391_m1 |
| Fgf10 (qPCR primer) | Thermo Fisher Scientific | Mm00433275_m1 |
| Wnt7b (qPCR primer) | Thermo Fisher Scientific | Mm00437358_m1 |
| Bmp4 (qPCR primer) | Thermo Fisher Scientific | Mm00432087_m1 |
| Shh (qPCR primer) | Thermo Fisher Scientific | Mm00436528_m1 |
| β-glucuronidase (qPCR primer) | Thermo Fisher Scientific | Mm00446953_m1 |
| Probes, Branches, Hairpins for SABER-FISH, see Table S1 | This paper | N/A |
| Software and algorithms | ||
| Zen blue | Zeiss | https://www.zeiss.com/ |
| Adobe Photoshop Creative Suite 3 | Adobe | https://www.adobe.com/ |
| Graphpad Prism 9.3.1 | Graphpad | https://www.graphpad.com/ |
| LASX | Leica | https://www.leica-microsystems.com/ |
| Other | ||
| Tamoxifen rodent diet (400mg/kg tamoxifen citrate) | Harlan Teklad | Cat# TD.130860 |