Table 1:
Kojak identified DSS-crosslinked CSMs at PeptideProphet/iProphet estimated <1% error rate (CSM-level).
| 1% Estimated Error Rate (CSM-level) | ||||||
|---|---|---|---|---|---|---|
|
| ||||||
| Correct (CSMa) | Incorrect (CSM) | Error Rate (CSM) | Correct (XLb) | Incorrect (XL) | Error Rate (XL) | |
| R1 | 405 | 2 | 0.49% | 156 | 2 | 1.27% |
| R2 | 539 | 3 | 0.55% | 166 | 2 | 1.19% |
| R3 | 490 | 1 | 0.20% | 165 | 1 | 0.60% |
| Total | 1434 | 6 | 0.42% | 182 | 3 | 1.62% |
a
Results are evaluated for each CSM above the 0.9 probability threshold.
b
Multiple CSMs to the same crosslinked pair of peptides are combined and results are evaluated for each unique crosslinked peptide pair.