Table 2:
Additional Kojak CSMs identified from DSSO cleavable crosslinker analysis at PeptideProphet/iProphet estimated <1% error rate (CSM-level).
| 1% Estimated Error Rate (CSM-level) | ||||||
|---|---|---|---|---|---|---|
|
| ||||||
| Correct (CSMa) | Incorrect (CSM) | Error Rate (CSM) | Correct (XLb) | Incorrect (XL) | Error Rate (XL) | |
| DSSO | 760 | 3 | 0.39% | 88 | 3 | 3.30% |
| DSSO, Cleavable | 2261 | 37 | 1.61% | 216 | 13 | 5.68% |
a
Results are evaluated for each CSM above the 0.9 probability threshold.
b
Multiple CSMs to the same crosslinked pair of peptides are combined and results are evaluated for each unique crosslinked peptide pair.