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Setup of an ultra-low attachment 96-well V-bottom plate for virus transfection of salivary gland epithelial buds.
| Wells | Usage | μL of medium to fill |
|---|---|---|
| A1-H1 & A12-H12 | edge wells to fill in medium to prevent evaporation | 150 |
| A2-A11 | virus 1 treatment | 0 |
| B2-C11 | virus 1 washes | 150 |
| D2-D11 | culture of virus 1 treated buds | 47 |
| E2-E11 | culture of virus 2 treated buds | 47 |
| F2-G11 | virus 2 washes | 150 |
| H2-H11 | virus 2 treatment | 0 |
