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RV-like PEG-PDHA NPs characterization. a) TEM images of RV-like NPs, scale bar = 10 nm. b) Hydrodynamic size distribution of PEG-PDHA NPs and RV-like NPs. c) PEG-PDHA NPs surface functionalization steps followed by ζ-Potential measurements at pH 7. d) Gel Electrophoresis of ASCL1-gRNA laden into PEG-PDHA NPs. Well 1: 1 kb Plus DNA ladder, well 2: Low DNA mass ladder, well 3: ASCL1-gRNA (8.3 kb), well 4: PEG-PDHA NPs and well 5: RV-like NPs.
