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. 2023 May 5;14(16):1477–1491. doi: 10.1111/1759-7714.14891

FIGURE 6.

FIGURE 6

TSC2 is the direct target of miR‐148b‐3p in macrophages. (a, b) qRT‐PCR and WB assays were carried out to evaluate TSC2 expression in macrophages after incubated with ectopic miR‐148b‐3p mimic or inhibitor. (c, d) qRT‐PCR and western blot assays were done to determine TSC2 expression in macrophages after incubated with TSC2‐overexpression plasmids. “TSC2‐OE” refers to “macrophages transfected with TSC2‐overexpression plasmids”. (e) Sequencing of the wild‐type and mutant target sites in 3′UTR of TSC2 mRNA. (f) Luciferase reporter activity was measured to verify the target association between miR‐148b‐3p and TSC2. The student's t‐test was adopted to analyze the statistical significance of the difference between two groups, and one‐way ANOVA test was utilized to analyze multiple groups (*p < 0.05, **p < 0.01, ***p < 0.001).