. 2023 Apr 13;54(2):1065–1073. doi: 10.1007/s42770-023-00969-5

Table 1.

Target genes/mutation, primer sequence, annealing temperature (°C), amplicon size, and reference of the primers used in this study

Gene	Primer sequence (5′-3′)	Sense	Annealing temperature	Size (pb)	Reference
cmeA	TTTGGATCCTTGATGGCTAAGGCAACTTTC	Forward	54°	771	[25]
cmeA	CTCCAATTTCTTAAGCTTCGCTACCAA	Reverse		771
cmeB	GGTACAGATCCTGATCAAGCC	Forward		820
cmeB	AGGAATAAGTGTTGCACGGAAATT	Reverse		820
cmeC	GCTTGGATCCTTATCTTGGGAAAAA	Forward		624
cmeC	TTTTTAAAGCTTTAAGGTAATTTTCTT	Reverse		624
ermB	GGGCATTTAACGACGAAACTGG	Forward	55°	421	[13]
ermB	CTGTGGTATGGCGGGTAAGT	Reverse	55°	421	[13]
gyrA	TTTTTAGCAAAGATTCTGAT	Forward	50°	265	[23]
gyrA	CAAAGCATCATAAACTGCAA	Reverse	50°	265	[23]
23sRNA	TTAGCTAATGTTGCCCGTACCG	Forward	59°	485	[24]
	TAGTAAAGGTCCACGGGGTCGC*	Reverse
	AGTAAAGGTCCACGGGGTCTGG**	Reverse
L4	TTATCCCTCTTTTGTAATAGATTCTAA	Forward	51°	614	[14]
L4	ATGAGTAAAGTAGTTGTTTTAAATGAT	Reverse	51°	614
L22	TTAGCTTTCCTTTTTCACTGTTGCTTT	Forward	55°	425
	ATGAGTAAAGCATTAATTAAATTCATAAG	Reverse
	TGAGAAGTTAAGTTTTGGAGAG	Reverse
SVR-flaA	CTATGGATGAGCAATTWAAAAT	Forward	60°	402	[26]
SVR-flaA	CAAGWCCTGTTCCWACTGAAG	Reverse	60°	402	[26]

^*Mutation detection position 2074, **mutation detection position 207