Table 3.
Strains, state, year of isolation, minimal inhibitory concentration (MIC) for ciprofloxacin (CIP) and erythromycin (ERY), molecular mechanisms associated with antimicrobial resistance and flaA alleles of 29 Campylobacter jejuni strains isolated from Broiler Carcasses in southern, Brazil
| Strain | State | Year of isolation | MIC CIP | Thr- 86-Ile | MIC ERY | 23S A2074C | 23S A2075G | ermB | L4 | L22 | cmeABC | flaA Allele |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cj80 | SC | 2018 | 16 | Positive | > 128 | Negative | Positive | Negative | WT | WT | Positive | 5 |
| Cj81 | SC | 2018 | 16 | Positive | > 128 | Positive | Positive | Negative | WT | WT | Positive | 5 |
| Cj89* | PR | 2018 | ≤ 0.125 | Negative | 2 | Negative | Negative | Negative | WT | WT | Positive | 16 |
| Cj86 | PR | 2018 | 16 | Positive | > 128 | Positive | Positive | Negative | T177S | WT | Positive | 34 |
| Cj88 | PR | 2018 | 16 | Positive | > 128 | Positive | Positive | Negative | T177S | WT | Positive | 34 |
| Cj23 | RS | 2017 | 16 | Positive | > 128 | Negative | Positive | Negative | T177S | WT | Positive | 34 |
| Cj43* | SC | 2017 | ≤ 0.125 | Negative | 0.5 | Negative | Negative | Negative | NT | NT | Positive | 36 |
| Cj7* | PR | 2017 | ≤ 0.125 | Negative | 1 | Negative | Negative | Negative | NT | NT | Positive | 45 |
| Cj32 | RS | 2017 | 32 | Positive | > 128 | Positive | Positive | Negative | T177S | WT | Positive | 54 |
| Cj33 | RS | 2017 | 32 | Positive | > 128 | Positive | Positive | Negative | T177S | WT | Positive | 54 |
| Cj50 | RS | 2018 | 32 | Positive | > 128 | Positive | Positive | Negative | WT | I65V/S109A | Positive | 54 |
| Cj90 | RS | 2018 | 32 | Positive | > 128 | Negative | Positive | Negative | WT | I65V/S109A | Positive | 54 |
| Cj93 | RS | 2018 | 8 | Positive | > 128 | Positive | Positive | Negative | WT | WT | Positive | 243 |
| Cj10 | SC | 2017 | 8 | Positive | > 128 | Positive | Positive | Negative | WT | WT | Positive | 287 |
| Cj26 | RS | 2017 | 32 | Positive | > 128 | Negative | Positive | Negative | WT | WT | Positive | 287 |
| Cj31 | SC | 2017 | 16 | Positive | > 128 | Positive | Positive | Negative | WT | I65V/S109A | Positive | 287 |
| Cj53 | SC | 2018 | 8 | Positive | > 128 | Positive | Positive | Negative | T177S | WT | Positive | 287 |
| Cj54 | RS | 2018 | 16 | Positive | > 128 | Positive | Positive | Negative | WT |
I65V/ A103V/ S109A |
Positive | 287 |
| Cj55 | RS | 2018 | 32 | Positive | > 128 | Positive | Positive | Negative | WT | I65V/ A103V/ S109A | Positive | 287 |
| Cj57 | RS | 2018 | 32 | Positive | > 128 | Negative | Positive | Negative | WT | I65V/ A103V/ S109A | Positive | 287 |
| Cj59 | RS | 2018 | 16 | Positive | > 128 | Positive | Positive | Negative | WT | I65V/ A103V/ S109A | Positive | 287 |
| Cj77 | RS | 2018 | 8 | Positive | 64 | Positive | Positive | Negative | WT | I65V/ S109A | Positive | 287 |
| Cj12* | PR | 2017 | ≤ 0.125 | Negative | 2 | Negative | Negative | Negative | NT | NT | Positive | 496 |
| Cj25 | SC | 2017 | 32 | Positive | > 128 | Negative | Positive | Negative | WT | I65V/ A103V/ S109A | Positive | 652 |
| Cj30 | SC | 2017 | 32 | Positive | > 128 | Negative | Positive | Negative | WT | I65V/ S109A | Positive | 975 |
| Cj37 | SC | 2017 | 32 | Positive | > 128 | Negative | Positive | Negative | WT | I65V/ S109A | Positive | 975 |
| Cj58 | RS | 2018 | 16 | Positive | 64 | Negative | Positive | Negative | WT | I65V/ S109A | Positive | 975 |
| Cj87 | RS | 2018 | 32 | Positive | > 128 | Positive | Positive | Negative | T177S | WT | Positive | 975 |
| Cj72* | PR | 2018 | ≤ 0.125 | Negative | 1 | Negative | Negative | Negative | NT | NT | Positive | 1123 |
*Sensitive strains to both antimicrobials. NT, non-tested; WT, wild type; PR, Paraná; SC, Santa Catarina; RS, Rio Grande do Sul