Table 1.

Synergistic effects of TNF-α and IFNg on barrier function evaluated by TEER.

Donor A, 72H: IFNγ EC50
TNFα [ng/mL]	0	0.14	0.41	1.2	3.7	11	33	100
IFNγ EC50 [U/mL]	Unstable	18	59	4.5	16	0.96	0.21	0.33
R2	0.554	0.782	0.937	0.905	0.996	0.997	0.981	1

Donor A, 72 H: TNFα EC50
IFNγ [U/mL]	0	1.6	8	40	200	1000
TNFα EC50 [ng/mL]	0.14	10	1.7	0.03	0.12	0.18
R2	0.430	0.941	0.845	0.676	0.801	0.902

Non-linear three parameter fit was used to calculate EC₅₀ values, shown with R² values which correspond to Fig. 3C plots. “Unstable” was designated by Prism software and indicates that no inflection point was defined.