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. 2023 Jun 1;13:8904. doi: 10.1038/s41598-023-34418-y

Figure 6.

Figure 6

MEF2A was identified to be a transcriptional activator of TLSP. (A, B) UCSC and JASPAR websites were employed to predict the binding site between TSLP promoter and MEF2A. (C, D) ChIP and dual-luciferase reporter gene assays were employed to analyze the interaction between MEF2A and the TSLP promoter. (E) MEF2A and TSLP mRNA levels in HaCaT cells after MEF2A knockdown/overexpression were assessed using RT-qPCR. (F) MEF2A mRNA level in serum and skin samples from AD mice and normal mice was assessed by RT-qPCR (n = 15). (G) Expression correlation of MEF2A and TSLP in serum and skin tissues of AD mice was analyzed by Spearman correlation analysis. Data were expressed as mean ± SD. All our data were obtained from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.