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. 2023 Jun 1;14:3175. doi: 10.1038/s41467-023-38789-8

Fig. 2. CNT1 kidney immunohistochemistry and targeted metabolomics of Slc28a1−/− mice urine.

Fig. 2

A Diagram illustrating transporter localization in the kidney. PTC, proximal tubular cell; arrows indicate transport directionality of CNT1 and ENT1. B Representative CNT1 DAB-stained kidney section (×10; Scale bar = 100 μm) comparing Slc28a1+/+ with Slc28a1−/− mice at 8 weeks. Insets shows higher magnification (×40; Scale bar = 20 μm) of boxed regions. CNT1 DAB staining is present at the apical surface of proximal tubule cells and glomerular cells (red arrows) in Slc28a1+/+ mice but not in the Slc28a1−/− mice. Kidney imaging performed three independent experiments. C Schematic workflow used to profile the nucleobases, nucleosides, and (deoxy)nucleosides in Slc28a1+/+ and Slc28a1−/− mice urine. D Concentration of nucleosides in Slc28a1−/− mice urine compared to Slc28a1+/+ littermate control mice. Data represent mean ± SEM (n = 6 mice/group, *p < 0.05 by two-tailed t-test). E Concentration of (deoxy)nucleosides in Slc28a1−/− mice urine compared to Slc28a1+/+ littermate control mice. Data represent mean ± SEM (n = 6 mice/group, *p < 0.05 by two-tailed t-test). F Concentration of nucleobases in Slc28a1−/− mice urine compared to Slc28a1+/+ littermate control mice. Data represent mean ± SEM (n = 6 mice/group, *p < 0.05 by two-tailed t-test). Profiling of nucleobases, nucleosides, and (deoxy)nucleosides was performed in two independent experiments.