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. 2023 Jun 1;10:326. doi: 10.1038/s41597-023-02204-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Assignment of the Metabarcoding samples against the negative controls database. Samples and negative controls (NC) sequences are clustered separately. NC clusters are assigned against SILVA databases. An OTU table is generated for each NC and a database from the NC clusters is generated. The sample clusters are assigned against these databases. OTU tables are generated and statistics are generated and visible through the NGL-BI web interface.