Table 1.
Overview of the Tara Pacific protocols (EUK for Eukaryote and PROK for Prokaryote).
| SAMPLE MATERIAL | SAMPLE PROTOCOL | Mainly targeted organisms | Sections | Targeted Genomic analysis | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Metagenomics | 16S metabarcoding | 18S metabarcoding | ITS2 metabarcoding | Dual-transcriptomics on RNA of EUK | Metatranscriptomics on RNA of EUK | Metatranscriptomics on RNA of PROK | Metaviromics | ||||
| MARINE | S300 (>300 µm) | Microplankton (EUK) | 2.1.1/2.2.1/3/4.1/5.2/6/7 | X | X | X | X | ||||
| MICROBIOME | S20 (>20 µm) | Microplankton (EUK) | X | X | X | X | |||||
| S320 (3–20 µm) | Nanoplankton (PROK,EUK) | X | X | X | X | X | |||||
| S3 (>3 µm) | |||||||||||
| S023 (0.2–3 µm) | Picoplankton (PROK,EUK) | 2.1.1/2.2.1/3/4.1/5/6/7 | X | X | X | X | X | ||||
| S<02 (<0.2 µm) | Viruses | 2.2.5/4.2/7 | X | ||||||||
| CORAL HOLOBIONT | CS4L | Millepora, Pocillopora, Porites, associated Symbiodiniaceae and microbiome (PROK,EUK) | 2.1.2/2.2.2/3/4.1/5.1/6/7 | X | X | X | X | X | |||
| CDIV | Diverse species of coral, associated Symbiodiniaceae and microbiome (PROK,EUK) | 2.1.2/2.2.3/3/6/7 | X | X | X | ||||||
| FISH | GT (Gut tissue) | associated microbiome (PROK,EUK) | 2.1.2/2.2.4/3.1/4.1/6/7 | X | X | ||||||
| MUC (Mucus tissue) | associated microbiome (PROK,EUK) | 2.1.2/2.2.4/3.1/6/7 | X | ||||||||
| AEROSOLS | AS-ABS | Marine aerosol (PROK,EUK) | 2.2.6/3.1/6/7 | X | |||||||
| SEDIMENT | SSED | Benthic microbiome (PROK,EUK), Symbiodiniaceae | 2.2.7/3.1/6/7 | X | X | X | |||||