Table 3.

Metabarcoding amplicon primers and expected PCR product lengths.

Target	Primer set name	Primer set sequences	Expected Length (bp)
16SFL	27 F/1492 R	5′-AGAGTTTGATCMTGGCTCAG–3′	1400
		5′-TACGGYTACCTTGTTACGACTT–3′
16SV4V5	515F-Y/926 R	5′-GTGYCAGCMGCCGCGGTA A-3′	411–600
		5′-CCGYCAATTYMTTTRAGTTT-3′
ITS2	SYM_VAR_5.8S2/SYM_VAR_REV	5′-GAATTGCAGAACTCCGTGAACC-3′	234–266
		5′-CGGGTTCWCTTGTYTGACTTCATGC 3′
18SV9	1389 F/1510 R	5′-TT GTA CAC ACC GCC C-3′	150–200
		5′-CCTTCYGCAGGTTCACCTAC-3′