Table 4.
Expected sequencing library size distribution (BioAnalyzer profiles).
| Library type | Library protocol | Expected size (bp) | Size Profil example |
|---|---|---|---|
| Metabarcoding 16SV4V5 | NebNext DNA-seq | 520–600/720–1000 |  |
| Metabarcoding ITS2 | NebNext DNA-seq | 420–500 |  |
| Metabarcoding 18SV9 | NebNext DNA-seq | 270–330 |  |
| Metagenomic | NebNext Ultra II DNA-seq | 300–800 |  |
| Metagenomic | NebNext DNA-seq | 300–800 |  |
| Dual-Transcriptomic Metatranscriptomic | TruSeq Stranded mRNA | 200–600 |  |
| Dual-Transcriptomic Metatranscriptomic | NebNext Ultra II RNA-seq oligo dT | 300–800 |  |
| Metatranscriptomic | SMARTer Stranded RNA | 200–600 |  |