. 2023 Feb 22;54(2):1035–1046. doi: 10.1007/s42770-023-00920-8

Table 3.

Phenotypic and genotypic characteristics of heat resistance in E. coli isolated from pasteurized milk samples obtained over 5 weeks from two producers from Mato Grosso, Brazil

Week	Isolate ID	*Heat resistance classification	D_{60 °C} (min)	tLST genes
4	^AA19C6	High	13.75	+
	^AA20C3	Sensitive	1.17	+
	^AA20C6	Moderate	5.95	+
5	^AA21C1	High	17.62	+
	^AA22C1	High	16.34	+
	^AA24C1	High	14.43	+
	^AA25C2	High	17.11	+
1	^BB01C3	Moderate	5.17	+
	^BB02C1	Moderate	5.07	+
	^BB03C4	Moderate	4.13	+
	^BB04C1	High	8.28	+
	^BB05C2	Moderate	4.11	+
2	^BB06C5	Moderate	6.91	+
	^BB07C2	Moderate	5.73	+
	^BB08C3	Moderate	7.69	+
	^BB10C2	Moderate	5.75	+
3	^BB11C1	Moderate	6.88	+
	^BB12C1	Moderate	5.21	+
	^BB13C1	Moderate	5.37	+
	^BB14C1	Moderate	4.23	+
	^BB15C1	Moderate	6.40	+
4	^BB16C2	Moderate	5.77	+
	^BB17C1	Moderate	5.07	+
	^BB18C2	Moderate	6.61	+
	^BB19C1	Moderate	7.00	+
	^BB20C3	Moderate	2.15	-
5	^BB21C1	Moderate	6.88	+
	^BB22C1	Moderate	11.00	+
	^BB23C2	Moderate	3.95	+
	^BB24C2	Moderate	6.00	+
	^BB25C1	Moderate	4.10	+

^AE. coli isolate from producer A; ^BE. coli isolate from producer B; presence or absence of transmissible locus of stress tolerance (tLST) genes. The heat resistance classification and D_60°C values were obtained with three independent replicates, each containing duplicate samples (3 × 2)