Fig. 1.

PCR amplification targeting IS407-fliP for Burkholderia mallei isolates from Brazil. All reactions shown in the figure are PCRs from microbiological cultures. Only one positive sample per animal was included. Arrow: 528 bp. Lane 1: 1 kb plus (Thermo Fisher, USA); lane 2: positive control: DNA from B. mallei (São Paulo) strain 86/19; lane 3: negative control; lane 4: Santa Catarina, equine, male, spleen; lane 5: Santa Catarina, equine, female, lung; lane 6: Bahia, equine, male, spleen; lane 7: Bahia, equine, male, lung; lane 8: Bahia, equine, female, tracheobronchial lymph node; lane 9: Bahia, equine, female, liver; lane 10: Bahia, equine, female, palate swab; lane 11: Rio Grande do Sul, equine, female, nasal swab; lane 12: Rio Grande do Sul, equine, male, mandibular lymph node; lane 13: Rio Grande do Sul, equine, male, ethmoidal concha abscess/piogranuloma; lane 14: Tocantins, mule, female, liver; lane 15: Mato Grosso, asinine, female, kidney; lane 16: Mato Grosso, equine, female; bladder; lane 17: Amazonas, equine, female, lung; lane 18: Piauí, equine, female, lymph node pool; lane 19: Sergipe, mule, female, liver; lane 20: Maranhão, equine, female, pulmonary lymph node