Table 2.

Results of PCR directly on tissues and colony PCR for Burkholderia mallei in equids from different geographical regions of Brazil

State	Species	Sample	Macroscopic lesions	Colony PCR	Tissue PCR
Rio Grande do Sul	Equine	Nasal swab	Not applicable	Positive	Not applicable
		Lung	Nodules	Negative	Negative
		Spleen	Nodules	Negative	Negative
		Liver	Nodules	Negative	Negative
		Mesenteric lymph node	No	Negative	Negative
		Retropharyngeal lymph node	No	Negative	Negative
		Deep cervical lymph node	Lymph node enlargement	Negative	Negative
		Pulmonary accessory lobe	No	Negative	Negative
		Mandibular lymph node	No	Negative	Negative
		Mediastinal lymph node	Lymph node enlargement	Negative	Negative
		Pulmonary lymph node	No	Negative	Negative
		Tracheobronchial lymph node	No	Negative	Negative
		Mandibular lymph node	No	Positive	Negative
		Spleen	Nodules	Positive	Negative
		Liver	Nodules	Positive	Negative
		Lung	Nodules	Negative	Negative
		Deep cervical lymph node	No	Negative	Negative
		Mediastinal lymph node	No	Negative	Negative
		Parotid lymph node	No	Negative	Negative
		Retropharyngeal lymph node	No	Negative	Negative
		Mesenteric lymph node	No	Negative	Negative
		Pulmonary accessory lobe	No	Negative	Negative
		Parotid	No	Negative	Negative
		Nasal swab	Not applicable	Negative	Not applicable
		Ethmoidal concha	Pyogranuloma or abscess	Positive	Positive
Santa Catarina	Equine	Liver	Pyogranuloma or abscess	Positive	Negative
		Spleen	Pyogranuloma or abscess	Positive	Negative
		Lung	Pyogranuloma or	Negative	Negative
			abscess
		Pulmonary lymph node	No	Negative	Negative
		Lung	Small pneumonic foci	Positive	Negative
		Liver	Mineralized surface lesion	Negative	Negative
		Kidney	No	Negative	Negative
		Lung	Nodules	Negative	Negative
		Pulmonary lymph node	No	Negative	Negative
		Spleen	Nodules	Negative	Negative
		Liver	Nodules	Negative	Negative
São Paulo	Equine	Trachea	Purulent tracheitis	Negative	Positive
		Tracheal swab	Not applicable	Positive	Not applicable
		Deep cervical lymph node	No	Negative	Negative
		Mesenteric lymph node	No	Negative	Negative
		Lung	Pyogranuloma or abscess	Negative	Positive
		Spleen lymph node	No	Negative	Negative
		Spleen	Pyogranuloma or abscess	Negative	Negative
		Heart	No	Negative	Negative
		Liver	Pyogranuloma or abscess	Negative	Positive
		Renal lymph node	No	Negative	Positive
		Kidney	No	Negative	Negative
Bahia	Equine	Spleen	Pyogranuloma or abscess	Positive	Negative
		Lung	Pyogranuloma or abscess	Positive	Negative
		Liver	Pyogranuloma or abscess	Negative	Negative
		Liver	Pyogranuloma or abscess	Positive	Negative
		Spleen	Pyogranuloma or abscess	Positive	Negative
		Lung	Pyogranuloma or abscess	Negative	Negative
		Tracheobronchial lymph node	Pyogranuloma or abscess	Positive	Negative
		Lung	Pyogranuloma or abscess	Negative	Negative
		Liver	Pyogranuloma or abscess	Negative	Negative
		Liver	Pyogranuloma or abscess	Positive	Negative
		Spleen	Pyogranuloma or abscess	Negative	Negative
		Lung	Multiple hemorrhagic foci	Negative	Negative
		Nasal swab	Not applicable	Positive	Not applicable
		Palate swab	Not applicable	Positive	Not applicable
		Mandibular lymph node	Pyogranuloma or abscess	Positive	Negative
		Lung	Focal pneumonia	Positive	Negative
		Pulmonary lymph node	Pyogranuloma or abscess	Negative	Negative
		Spleen	Petechiae	Negative	Negative
		Liver	Pyogranuloma or abscess	Negative	Negative
Piauí	Equine	Pool of pulmonary and mediastinal lymph nodes	No	Positive	Negative
		Nasal swab	Not applicable	Positive	Not applicable
		Tracheal swab	Not applicable	Negative	Not applicable
Sergipe	Mule	Liver	Pyogranuloma or abscess	Positive	Negative
		Lung	Pyogranuloma or abscess	Negative	Negative
		Liver fluid	Not applicable	Negative	Negative
		Mesenteric lymph node	Lymph node enlargement	Negative	Positive
		Lung fluid	Not applicable	Negative	Negative
Maranhão	Equine	Pulmonary lymph node	Nodules and pyogranuloma or abscess	Positive	Negative
		Duodenal pancreatic lymph node	No	Negative	Negative
		Lymph node	No	Negative	Negative
		Liver	Nodules and pyogranuloma or abscess	Negative	Negative
		Tracheal swab	Not applicable	Negative	Not applicable
		Nasal swab	Not applicable	Negative	Not applicable
Tocantins	Mule	Liver	Pyogranuloma or abscess	Positive	Negative
		Lung	No	Negative	Negative
Amazonas	Equine	Lung	Nodules	Positive	Negative
		Prescapular lymph node	No	Negative	Negative
		Liver	Nodules	Negative	Negative
Mato Grosso	Asinine	Parotid lymph node	Nodules	Positive	Negative
		Palate	No	Positive	Negative
		Kidney	Pyogranuloma or abscess	Positive	Positive
		Lung	No	Positive	Negative
		Mesenteric lymph node	No	Negative	Negative
		Sublingual lymph node	No	Negative	Negative
		Spleen	Pyogranuloma or abscess	Negative	Negative
		Adrenal	No	Negative	Negative
		Prescapular lymph node	Nodules	Negative	Negative
		Mediastinal lymph node	Nodules	Negative	Negative
		Subiliac lymph node	No	Negative	Negative
		Brain	No	Negative	Negative
		Liver	No	Negative	Negative
		Nasal sinus	No	Negative	Negative
	Equine	Lung	Pyogranuloma or abscess	Positive	Negative
		Bladder	No	Positive	Negative
		Liver	Pyogranuloma or abscess	Negative	Negative
		Mediastinal lymph node	No	Negative	Negative
		Kidney	No	Negative	Negative
		Heart	No	Negative	Negative
		Superficial cervical lymph node	No	Negative	Negative
		Spleen	No	Negative	Negative