FIGURE 14.

Red light pulse arousal behavior is significantly attenuated in flies lacking external photoreceptors at low and high light intensity compared to the control p12c. Three 5 min pulses of red light were applied to flies during subjective nighttime (ZT18, ZT19, ZT20) for 3 days after 12:12 h LD entrainment to measure the arousal response of p12c (blue), gl60j (red), cry-null (green), gl60j-cry-null (yellow), and rh7-null (violet) flies for (A) low (10 μW/cm²) and (B) high (400 μW/cm²) light intensity. Scatter plots are grouped by average% of flies that awaken across the 3 days of light-pulse arousal experiment and separated as pulse 1 (left points for each group), pulse 2 (middle points for each group), and pulse 3 (right points for each group). Black bars indicate total average% flies that awaken across the 3 days and three pulses of light. Pairwise comparison was analyzed using two-sample t-test with FDR adjustment. **p < 0.05 and ***p < 0.01.