Figure 7.

Pretreatment with anti-IL-1α Ab dampened δ-toxin-mediated, OVA-induced food allergic responses in murine model. (A) Experimental design for food allergy of intragastrically OVA-administered WT mice that had been epicutaneously treated with OVA + δ-toxin once a week for six weeks on the non-tape-stripped abdominal skin. The effects of intraperitoneal administration of anti-IL-1α Ab or control Ab were examined. Blood samples were taken, and small intestines were isolated on day 57 (B) Frequency of diarrhea in OVA-administered mice. (C–E) The serum levels of (C) OVA-specific IgE and (D) MCPT-1 in the mice after the last challenge with OVA. (E) The numbers of jejunum mast cells of the mice after the last challenge with OVA. (F) Jejunum sections stained with chloroacetate esterase (scale bars, 100 μm). Mast cells stain red. (B-F) Data are representative of two independent experiments. Means ± SD have been plotted. *P < 0.05 or **P < 0.01.