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. 2023 May 19;14:1175275. doi: 10.3389/fimmu.2023.1175275

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Copyright © 2023 Martins, de Oliveira, Souza, Queiroz-Junior, Lobo, Teixeira, Malacco and Soriani

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Alcohol-fed mice have decreased neutrophil and macrophage infiltration into airways 24 hours post pneumococcal infection. After alcohol treatment, mice were infected with S. pneumoniae. After 24h hours of infection, mice were euthanized, BALF, blood and lungs were collected to determine the infiltration of inflammatory cells. (A) Total cells, (B) Neutrophils and (C) Macrophages count in BALF. (D) Total cells, (E) polymorphonuclear cells and (F) mononuclear cells into the blood. (G) Neutrophils and (H) macrophages infiltrated into the lung parenchyma. Data are presented as the mean ± SD (7 to 11 mice per group). * Significantly different (p < 0.05), **Significantly different (p < 0.01), ***Significantly different (p < 0.001) by two-way ANOVA analysis test.