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. 2023 Jun 1;6(8):e202301902. doi: 10.26508/lsa.202301902

Figure 1. miR-33b KI mice exhibit the NASH phenotype under HFD feeding.

Figure 1.

(A) Scheme of the HFD feeding protocol. (B) Representative microscopic images of HE staining of the liver of miR-33b−/− and miR-33b+/+ mice fed the HFD. Scale bars: 300 μm (upper). (C) Total cholesterol and triglyceride levels in the livers of miR-33b−/− and miR-33b+/+ mice fed the HFD. n = 4 mice per group; **P < 0.01, unpaired t test. (D) Relative expression levels of miR-33 target genes in the livers of miR-33b−/− and miR-33b+/+ mice fed the HFD. n = 4–5 mice per group; **P < 0.01, unpaired t test. (E) Western blotting analysis of ABCA1, CPT1A, and CROT expression in the livers of miR-33b−/− and miR-33b+/+ mice fed the HFD. GAPDH was used as a loading control. n = 5 mice per group. (F) Densitometry of hepatic ABCA1, CPT1A, and CROT. n = 5 mice per group; *P < 0.05 and **P < 0.01, unpaired t test. (G) Relative expression levels of inflammatory genes and fibrosis-related genes in the livers of miR-33b−/− and miR-33b+/+ mice fed the HFD. n = 5–8 mice per group; **P < 0.01, unpaired t test. (H) Western blotting analysis of COL1A1 expression in the livers of miR-33b−/− and miR-33b+/+ mice fed the HFD. GAPDH was used as a loading control. n = 5 mice per group. (I) Densitometry of hepatic COL1A1. n = 5 mice per group; *P < 0.05, unpaired t test. (J) Representative microscopic images of Masson’s trichrome staining of the livers of miR-33b−/− and miR-33b+/+ mice fed the HFD. Scale bars: 100 μm.