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. 2023 Jun 1;6(8):e202301902. doi: 10.26508/lsa.202301902

Figure 2. HFD-induced NASH phenotype is ameliorated in Alb-Cre/miR-33bfl/fl KI mice.

Figure 2.

(A) Representative microscopic images of HE (upper) and Picro-Sirius red (lower) staining of the livers of miR-33bfl/fl KI and Alb-Cre/miR-33bfl/fl KI mice fed the HFD. Scale bars: 200 μm. (B) Relative expression levels of Abca1 and Cpt1a in the livers of miR-33bfl/fl KI and Alb-Cre/miR-33bfl/fl KI mice fed the HFD. n = 10–11 mice per group; ***P < 0.001, unpaired t test. (C) Western blotting analysis of ABCA1 and CPT1A expression in the livers of miR-33bfl/fl KI and Alb-Cre/miR-33bfl/fl KI mice fed the HFD. GAPDH was used as a loading control. n = 5 mice per group. (D) Relative expression levels of Col1a1, Acta2, and Tnf in the livers of miR-33bfl/fl KI and Alb-Cre/miR-33bfl/fl KI mice fed the HFD. n = 9–10 mice per group; *P < 0.05, unpaired t test. (E) Quantification of the fibrosis area in the liver sections of miR-33bfl/fl KI and Alb-Cre/miR-33bfl/fl KI mice fed NC or the HFD according to Picro-Sirius red staining. n = 6–7 mice per group; ***P < 0.001, one-way ANOVA with Tukey’s post hoc test. (F) Total cholesterol content in the livers of miR-33bfl/fl KI and Alb-Cre/miR-33bfl/fl KI mice fed NC or the HFD. n = 6–7 mice per group; *P < 0.05 and ***P < 0.001, one-way ANOVA with Tukey’s post hoc test. (G) Triglyceride content in the livers of miR-33bfl/fl KI and Alb-Cre/miR-33bfl/fl KI mice fed NC or the HFD. n = 6–7 mice per group; ***P < 0.001, one-way ANOVA with Tukey’s post hoc test.