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. 2023 Mar 8;14(3):1424–1440. doi: 10.1002/jcsm.13214

Figure 5.

Figure 5

Post‐septic satellite cell RNA seq reveals metabolic defects potential contributions to long term functional deficits. Combined experiment (A) principal component analysis (PCA) of control and cecal ligation and puncture/daily chronic stress (CLP/DCS) Days 4, 8 and 28 utilizing all gene transcriptomic data. (B) Hierarchical clustering by means of combined differentially expressed genes from CLP/DCS Days 4, 8 and 28 versus control using 2 < Log2FC < −2, P < 0.05. (C) Venn diagram illustrating the overlap in significantly altered genes in CLP/DCS Days 4, 8 and 28 samples versus control, where 2 < Log2FC < −2, P < 0.05. (D) Top 4 altered pathways from ingenuity pathway analysis (IPA) in CLP/DCS Day 28 versus control samples. Orange bars indicate z‐score, blue bars indicate ‐log(P‐value). (E) Heatmap of significantly altered oxidative phosphorylation genes in CLP/DCS Day 28 versus control (depicted as Log2 transcript expression [FPKM] of CLP/DCS Days 4, 8 and 28 as a fold change from control) that are represented in more than one of the top 4 altered pathways from IPA; Ndufa6, Ndufb8 transcript variant 1, Ndufb11, Sdhb, Sdhc, Sdhd, Cyc1, Uqcrc2, Cox4i1 transcript variant 1, Cox7b, Atp4d transcript variant 2 and Atp5e. Significance indicated is versus control. B = Clustering method: complete linkage; distance measure: correlation. E = One‐way ANOVA with Bonferroni multiple comparisons (BMC) whereby significance is *P < 0.05, **P < 0.01, ***P < 0.001. n = 4–5. Each n = pooled satellite cells isolated from 2–3 mice. Satellite cells harvested from mice on Day 27 or 28 are labelled as Day 28.