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. 2023 May 2;14(3):1395–1409. doi: 10.1002/jcsm.13245

Figure 5.

Figure 5

Faecal microbiota transplantation (FMT) of db/db mice fed normal diet with milk increased skeletal muscle mass and decreased the expression of genes related with muscle atrophy. (A) Representative images of haematoxylin and eosin‐stained soleus muscle sections. Soleus muscle tissues were collected at 16 weeks of age. The scale bar shows 100 μm. (B) Cross‐sectional area and diameter of soleus muscle (n = 6). (C) Absolute and relative soleus muscle weight, (D) absolute and relative plantaris muscle weight and (E) absolute and relative epididymal fat weight in 16‐week‐old mice (n = 6 in each case). (F) Relative mRNA expression of Foxo1, Mstn, Fbxo32 and Trim63 in the plantaris muscle normalized to the expression of Gapdh (n = 6). Data are represented as the mean ± SD values. Data were analysed using paired t‐test. BW, body weight. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.