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GHK‐Cu directly bound and activated SIRT1. (A) Process of target screening. (B) Interaction of potential targets. (C) Interactive sites between GHK‐Cu and SIRT1 by docking analysis. (D) GHK‐Cu increased SIRT1 expression dose‐dependently in CSE‐treated C2C12 myotubes. (E) GHK‐Cu enhanced SIRT1 deacetylase activity in C2C12 myotubes. ns, no significance; *P < 0.05; **P < 0.01; ***P < 0.001.
