Table 2. The benefits and negatives of the different ways to extract adipose tissue from bench to bedside.

Device	Method	Positive	Negative
Mechanical isolation (Lipogems and Lipocell)	Minimal invasive procedure (liposuction) usually fat tissue taken from the abdomen	Minimally invasive FDA approved Quick treatment Reduce need for major intervention Enzyme-free	Expensive
Enzymatic digestion	Lipo-aspiration	Efficient Yields higher cell counts	Expensive Prolongs the isolation time External compounds add (enzymes) Enzymatic method may be too aggressive and might destroy exosomes during processing Enzymatic treatment of cells digests the extracellular matrix surrounding the cells, possibly affecting cell secretory functions Might damage the cells, affecting cell function and viability