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Microtubule configurations in the distal axoneme. In demembranated cilia, microtubules rotate and separate in the distal portion of the axoneme, creating a helical cage (A-D). Occasional actin-like thin filaments are seen coursing along the microtubules (C). Splaying of individual microtubule filaments is likely due to specimen damage (E and F). Scale, A and B, 500 nm; C–F, 300 nm.
