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. 2023 May 22;120(22):e2300773120. doi: 10.1073/pnas.2300773120

Fig. 1.

Fig. 1.

The δ-catenin G34S mutation increases GSK3β-mediated δ-catenin degradation. (A) Representative immunoblots and summary graphs of normalized δ-catenin and pGSK3β levels in SH-SY5Y cell lysates transfected with WT or G34S δ-catenin and treated with 2 mM NaCl (−) or 2 mM LiCl (+) (n = number of immunoblots from five independent cultures. For δ-catenin, WT + NaCl = 14, WT + LiCl = 16, G34S + NaCl = 9, and G34S + LiCl = 17. For pGSK3β, WT + NaCl = 14, WT + LiCl = 16, G34S + NaCl = 11, and G34S + LiCl = 17. Two-way ANOVA with the Tukey test, *P < 0.05, **P < 0.01, and ****P < 0.0001). (B) Representative immunoblots and summary graphs of normalized δ-catenin and GSK3β levels in SH-SY5Y cell lysates transfected with WT or G34S δ-catenin and treated with scrambled (CTRL) (−) or GSK3β (+) siRNA (n = number of immunoblots from five independent cultures. For δ-catenin, WT + CTRL siRNA = 13, WT + GSK3β siRNA = 18, G34S + CTRL siRNA = 12, and G34S + GSK3β siRNA = 18. For GSK3β, WT + CTRL siRNA = 15, WT + GSK3β siRNA = 18, G34S + CTRL siRNA = 14, and G34S + GSK3β siRNA = 18. Two-way ANOVA with the Tukey test, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001). The position of molecular mass markers (kDa) is shown on the right of the blots. Mean ± SD.