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. 2023 May 22;120(22):e2302622120. doi: 10.1073/pnas.2302622120

Fig. 1.

Fig. 1.

Reconstitution of endocytic actin networks on supported lipid bilayers. (A) Schematic representation of the in vitro reconstitution assay. Purified scWASP (Las17) is attached to supported lipid bilayers, which are then incubated in yeast cytoplasmic extract, resulting in the assembly of endocytic actin networks. (B) GelCode-stained SDS-PAGE gel of purified scWASP (arrowhead). (C) Purified scWASP binds to supported lipid bilayers, as shown by western blot probed with an anti-Las17 antibody. (D) Sac6-RFP from yeast cytoplasmic extract labels actin networks assembled on scWASP-coated supported lipid bilayers (Atto647-DOPE, lipid). 180 µM latrunculin A (LatA) inhibits actin assembly on bilayers (Scale bar, 5 µm.).