Fig. 6.

Development of a molecular marker for genotyping the RsMs1 locus. A Primer positions of the molecular marker and copy number estimation of the 955-bp insertion. Gray and green boxes indicate flanking regions and 955-bp insertion, respectively. Arrows indicate primer-binding sites. Copy numbers of repeat sequences showing homology (E value < 1.0E-100) with the 955-bp insertion are depicted under the recessive allele organization. Repeat sequences were identified from the radish draft genome sequence produced by Jeong et al. (2016). B PCR products of two primer combinations. The RsMs-F1m is a modified primer containing a single mismatched nucleotide. Primer sequences are listed in Supplementary Table 2. A, homozygous dominant; H, heterozygous; B, homozygous recessive individuals