Fig. 2.

Using the CRISPR/Cas9 system for knockout of Ms10³⁵ and its marker genes. a The second exon of Ms10³⁵ and its marker genes were targeted by the CRISPR/Cas9 system using single-guide RNAs (red arrows indicate target). Black arrows indicate the forward (F) and reverse (R) primers used for PCR genotyping and sequencing. The target sequences in Ms10³⁵ and GSTAA are underlined, and minus symbols represent deletions. The numbers on the right show the type of mutation and how many nucleotides are involved, with “−”or “+” indicating the deletion or insertion of the given number of nucleotides, respectively. b Editing efficiency of CR-Ms10³⁵/Wo and CR-Ms10³⁵/GSTAA in T0 transgenic plants