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. 2023 May 15;26(1):316. doi: 10.3892/etm.2023.12015

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Copyright: © Qian et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effect of circDHTKD1 knockdown on cell inflammation response. (A) RT-qPCR analysis of circDHTKD1 following transfection of BEAS-2B cells with si-NC, si-circ#1 or si-circ#2 for 48 h. BEAS-2B cells were transfected with si-NC or si-circ#1 followed by treatment with 5 µg/ml lipopolysaccharide. mRNA expression of (B) IL-6, (C) IL-8, (D) TNF-α and (E) VEGF was measured by RT-qPCR. (F) ELISA was used to measure concentrations of IL-6 and VEGF. ^*P<0.05, ^**P<0.01. si-NC, small interfering RNA negative control; RT-qPCR, reverse transcription-quantitative PCR; LPS, lipopolysaccharide; DHTKD1, dehydrogenase E1; circ, circular.