Skip to main content
. 2023 Mar 28;13(6):1364–1385. doi: 10.1158/2159-8290.CD-22-1427

Figure 6.

Figure 6. Identification of a likely non–whole-genome–doubled clone that was not identifiable from bulk sequencing data in CRUKP2567. Clonal phylogeny of CRUKP2567 (A), with anatomic diagram (B) based on bulk SNVs mapping samples to clones on the tree. The scale indicates the number of mutations. C, MEDICC2 copy-number tree for bulk exome samples from CRUKP2567. The cluster highlighted in blue has undergone one WGD event, while the other nonhighlighted cluster, containing brain metastasis and primary tumor samples, has not. Diamonds indicate the samples for which bulk copy-number profiles are displayed in Supplementary Fig. S23. D, Hierarchical clustering tree containing all single cells (SS) from FACs-high-ploidy sorting (FH) and FACs-low-ploidy sorting (FL), as well as WGD bulk samples and non-WGD bulk samples. E, Radiologic images of the patient indicating thorax upon initiation of stage IV disease and complete extracranial response to BRAF inhibitor, followed by rapid recolonization of the thorax with resistant clones (left to right). BR, brain; BRAFi, BRAF inhibitor; CR, complete response; DI, diaphragm; LN, lymph node; LU, lung; MEKi, MEK inhibitor; PC, pericardium, PLFa, pleural fluid (archival); PR, primary.

Identification of a likely non–whole-genome–doubled clone that was not identifiable from bulk sequencing data in CRUKP2567. Clonal phylogeny of CRUKP2567 (A), with anatomic diagram (B) based on bulk SNVs mapping samples to clones on the tree. The scale indicates the number of mutations. C, MEDICC2 copy-number tree for bulk exome samples from CRUKP2567. The cluster highlighted in blue has undergone one WGD event, while the other nonhighlighted cluster, containing brain metastasis and primary tumor samples, has not. Diamonds indicate the samples for which bulk copy-number profiles are displayed in Supplementary Fig. S23. D, Hierarchical clustering tree containing all single cells (SS) from FACs-high-ploidy sorting (FH) and FACs-low-ploidy sorting (FL), as well as WGD bulk samples and non-WGD bulk samples. E, Radiologic images of the patient indicating thorax upon initiation of stage IV disease and complete extracranial response to BRAF inhibitor, followed by rapid recolonization of the thorax with resistant clones (left to right). BR, brain; BRAFi, BRAF inhibitor; CR, complete response; DI, diaphragm; LN, lymph node; LU, lung; MEKi, MEK inhibitor; PC, pericardium, PLFa, pleural fluid (archival); PR, primary.