Table 1.
Primer sequences and conditions for amplification of 16S rRNA and antibiotic resistance genes of E. tarda by PCR.
| Target Gene | Primer sequence (5`-3`) | Amplified segment (bp) | Initial denaturation | Final denaturation | Annealing | Initial extension | Final extension | Reference |
|---|---|---|---|---|---|---|---|---|
| blaTEM | F-CATTTCCGTGTCGCCCTTATTC R-CGTTCATCCATAGTTGCCTGAC |
800 | 95°C For 2 min | 95°C For 30 s 35 cycles | 52°C For 30 s 35 cycles | 72°C For 1min 35 cycles | 72°C For 7 min | [39] |
| qnrA | F-ATTTCTCACGCCAGGATTTG R-GATCGGCAAAGGTTAGGTCA |
516 | 95°C For 10 min | 95°C For 1min 35 cycles | 58.5°C For 1min 35 cycles | 72°C For 1min 35 cycles | 72°C For 10 min | [40] |
| sul3 | F-AGATGTGATTGATTTGGGAGC R-TAGTTGTTTCTGGATTAGAGCCT |
443 | 93°C For 3 min | 93°C For 30s 35 cycles | 54.2°C For 30 s 35 cycles | 72°C For 1min 35 cycles | 72°C For 7min | [39] |
| 16SrRNA gene | F-AGAGTTTGATCCTGGCTCAG R-GGTTACCTTGTTACGACTT |
1503 | 94°C For 5 min | 90°C For 30 s 30 cycles | 52°C For 1min 30 cycles | 72°C For 1min 30 cycles | 72°C for 8 min | [41] |
| gyrB | F-GCATGGAGACCTTCAGCAAT R-GCGGAGATTTTGCTCTTCTT |
414 | 94°C For 1 min | 94°C For 1min 32 cycles | 55°C For 1min 32 cycles | 72°C For 1min 32 cycles | 72°C For 10 min | [66] |