Table 1.
Physiological and biochemical characteristics of the isolate, BH35.
| Characteristics | Growth result | Characteristics | Growth result |
|---|---|---|---|
| (1) Melanin pigment production | (5) Growth at different temperature (°C) | ||
| Liquid tryptone yeast extract | + | 20 | + |
| Tyrosine agar | − | 30 | ++ |
| Peptone yeast extract iron agar | + | 33 | +++ |
| (2) Utilization of carbon sources | 40 – 45 | Wg | |
| Fructose | +++ | 50 | − |
| D-glucose | +++ | (6) Tolerance to NaCl (%) | |
| D-mannitol | ++ | 1 – 3 | +++ |
| L-arabinose | − | 4 | Wg |
| Inositol | − | 5 | − |
| L-rhamnose | − | (7) Degradation ability | |
| D-xylose | Wg | Citrate | ++ |
| Sucrose | +++ | Pectin | + |
| Cellulose | + | Tyrosine | − |
| Dextrin | Wg | Esculin | Wg |
| Glycerol | ++ | Xanthine | − |
| (3) Utilization of nitrogen sources | Urea | + | |
| L-tyrosine | − | (8) Tolerance to growth inhibitors (%) | |
| L-lysine | − | Crystal violet (0.0001) | + |
| L-arginine | ++ | Potassium tellurite (0.001) | − |
| L-asparagine | +++ | Sodium azide (0.01) | + |
| L-cysteine | − | Phenol (0.1) | − |
| L-histidine | + | (9) Sensitivity to antibiotics (μg) | |
| L-phenylalanine | + | Gentamicin (30) | S |
| L-tryptophan | +++ | Rifampicin (50) | S |
| (4) Growth at different pH values | Vancomycin (50) | R | |
| 5 | Wg | Streptomycin (50) | R |
| 6 | + | Penicillin G (10 IU) | R |
| 7 | + | (10) Other biochemical tests | |
| 8 | ++ | Nitrate reduction | + |
| 9 | ++ | H2S production | − |
| 10 | +++ | Amylase | + |
| 11 | + | Catalase | + |
| 12 | − | Lipase | + |
“−” Negative, “Wg” weak growth, “+” Moderate, “++” Good, “+++” Abundant growth, “R” Resistant, “S” Sensitive.