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SpCas9-specific T cells are not detected by IFN-γ ELISpot assay
(A) Outline of experiment performed on nine rhesus macaque PBMCs. (B) IFN-γ ELISpot performed on day 0. (C and D) IFN-γ ELISpot assay results performed on day 9 following stimulation with (C) SpCas9 or (D) rhCMV/HuAdV pepmixes. Saturated: complete confluency of spot-forming cells (SFC).
