Fig. 3.

Gel electrophoresis image (3a) of PCR products of Staphylococcus spp. showing specific amplified bands on 1.5% agarose gel, where lanes 1 and 2 = S. aureus (894 bp), 3 and 4 = S. sciuri (306 bp), 5 and 6 = S. chromogenes (222 bp), 7 and 8 = S. epidermidis (130 bp), and 9 and 10 = S. haemolyticus (214 bp). Image-3b and 3c display the specific amplification of the nuc (359 bp) and mecA (162 bp) genes, respectively. In addition, images 3d, 3e, and 3f exhibit the amplification of virulence genes like sea (102 bp), pvl (255 bp), and tst (326 bp), respectively. In all gel electrophoresis systems, we used a 1 kb plus DNA marker except for images 3b and 3c, where we used 100 bp DNA markers for confirmation of amplification of specific genes. Moreover, lanes 1 and 2 were used as positive and negative controls for the 3b, 3c, 3d, 3e, and 3f images, respectively.