Table 2.
Primer sequences used for nuc, mecA and virulence genes amplification.
| Organism | Gene Primer | Primer designation | Primer sequence 5´−3´ | Amplicon size (bp) | References |
|---|---|---|---|---|---|
| Staphylococcus spp. | nuc | nucF | TCGCTTGCTATGATTGTGG | 359 | Sasaki et al., 2010 |
| nucR | GCCAATGTTCTACCATAGC | ||||
| mecA | MecF | TCCAGATTACAACTTCACCAGG | 162 | Larsen et al., 2008 | |
| MecR | CCACTTCATATCTTGTAACG | ||||
| sea | SF | GGTTATCAATGTGCGGGTGG | 102 | Zhou et al., 2017 | |
| SR | CGGCACTTTTTTCTCTTCGG | ||||
| seb | SF | GTATGGTGGTGTAACTGAGC | 164 | ||
| SR | CCAAATAGTGACGAGTTAGG | ||||
| sec | SF | AGATGAAGTAGTTGATGTGTATGG | 451 | ||
| SR | CACACTTTTAGAATCAACCG | ||||
| tst | SF | ACCCCTGTTCCCTTATCATC | 326 | ||
| SR | TTTTCAGTATTTGTAACGCC | ||||
| eta | SF | ATATCAACGTGAGGGCTCTAGTAC | 1155 | ||
| SR | ATGCAGTCAGCTTCTTACTGCTA | ||||
| etb | SF | CACACATTACGGATAATGCAAG | 604 | ||
| SR | TCAACCGAATAGAGTGAACTTATCT | ||||
| pvl | SF | GTGCCAGACAATGAATTACCC | 255 | ||
| SR | TTCATGAGTTTTCCAGTTCACTTC |