Fig. 7.

The conditioned media (CdM) of cell-seeding SIS patches stimulate proliferation of cardiomyocytes (CMs) and functions of HUVECs. (A) Representative images and quantitative analysis of ki67⁺ CMs co-immunocytochemical stained for ki67 and α-actinin in the hCMs incubated with various CdM for 2 days. Scale bar, 50 μm (upper panels) and 10 μm (lower panels). n = 3 independent repeats. (B) Representative images and quantitative analysis of tube formation of HUVECs incubated with various CdM for 24 h. Scale bar, 500 μm. n = 5 independent repeats. (C) Views of the scratch induced in the HUVEC monolayer and wound invasion after incubation for 12 h with various CdM. Scale bar, 500 μm. All values were mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001; ns, no statistical significance.